SARS- and Other Coronaviruses: Laboratory Protocols

"In 2003, the word"coronavirus" spread across the globe, somewhat further than the viru that sparked the panic. In SARS and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis byelectron cryomicroscopy, expression and crystallization of virai proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls."--BOOK JACKET.Cavanagh, Dave is the author of 'SARS- and Other Coronaviruses: Laboratory Protocols', published 2008 under ISBN 9781588298676 and ISBN 1588298671.