Principles of RNAi

RNA interference is now one of the hottest topics in molecular biology and the most widely used method for determining gene function. However, in the rush to adopt this powerful technology, it is easy to overlook the basic principles of the technique and so make time-consuming mistakes.Principles of RNAi provides newcomers to the field with a comprehensive and authoritative guide to the technology, covering all the key background, including:?history and significance of RNA silencing'mechanism of RNA interference?RNA silencing as a biological and an evolutionary process'practical aspects of RNAi in vertebrates, plants, invertebrates, and microbes'research applications in biology, medicine and pharmacologyThis book describes the principles of the methodology, discusses what has worked and what has not in practice in the key research systems, and concludes with a concise summary of how the technology can be put to use. Principles of RNAi is essential reading for every molecular biologist wishing to understand and utilize this technique successfully in their research.________________________________________Contents:1. RNA silencing: history and significance2. The mechanism of RNA interference 3. RNA silencing as a biological process - microRNAs and gene regulation4. RNA silencing and the structure of DNA and chromatin 5. RNA silencing as an evolutionary process ? regulation and defence6. Practical aspects of RNAi in C. elegans, Drosophila and other invertebrates7. Practical aspects of RNAi in plants8. Practical aspects of RNAi in vertebrates9. Practical aspects of RNAi in microbes10. Applications of RNA silencingTwyman, Richard is the author of 'Principles of RNAi', published 2008 under ISBN 9781904842255 and ISBN 1904842259.