PCR

PCR is probably the most widely-used technique in molecular biology today, with new variants appearing on a frequent basis. Many of the new methods offer substantial benefits over protocols that have previously been covered in original papers and lab manuals. PCR: Methods Express describes the latest PCR-based methodologies and approaches and provides the most up-to-date practical advice on how to tackle a broad range of biological problems using these techniques.PCR: Methods Express is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategies for optimization. This book is an essential laboratory manual for researchers in all life science fields, and at all levels from postgraduate student to principal investigator.________________________________________Contents:1. An introduction to the polymerase chain reaction Adrian Moody, AstraZeneca2. Polymerases for PCR Meg Martel, Simon Baker and Simon May, ABGene3. A detailed guide to quantitative RT-PCR Pete Kaiser, Institute for Animal Health, Compton, Newbury4. Use of quantitative PCR for the detection of genomic microdeletions or microduplications Simon Hughes, Tumour Biology Laboratory, London; Rosanna Weksberg, The Hospital for Sick Children, Toronto; Jeremy Squire, Ontario Cancer Institute, Toronto 5. Robust and unique PCR for single-nucleotide polymorphism genotyping applications Xiangning Chen, Virginia Institute for Psychiatric and Behavioral Genetics 6. Using PCR and linkage mapping to identify single genes and quantitative trait loci for livestock traits Jill Maddox, Sonja Dominik and Imke Tammen, all Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne 7. PCR restriction fragment length polymorphism analysis for genotyping of single-nucleotide polymorphisms Simon Hughes, Tumour Biology Laboratory, London8. Forensic genetic DNA typing with PCR-based methods Claus B'rsting, Juan Sanchez and Niels Morling, all at the Institute of Forensic Medicine, K'benhavns University9. Large PCR multiplexes with special reference to forensic single-nucleotide polymorphism typing Juan Sanchez, Claus B'rsting and Niels Morling, all at the Institute of Forensic Medicine, K'benhavns University10. Pre-implantation genetic diagnosis of monogenic disease: PCR-based methods for the identification of mutations in single cells Dagan Wells, Yale University Medical School 11. Rapid generation of gene-targeting constructs Trevor Wilson, Antonietta Giudice and Paul Hertzog, all at the Monash Institute of Medical Research12. Construction of long DNA molecules from multiple fragments using PCR Nikolai Shevchuk and Anton Bryksin, Center for Cancer and Immunology Research, Washington13. Efficient PCR-based mutagenesis method applicable to diverse mutagenesis strategies using type IIs restriction enzyme Jae-Kyun Ko and Jianjie Ma, both at Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey 14. Inverse PCR-based restriction fragment length polymorphism for identifying low-level mutations in tumors Mike Makrigiorgos, Dana Farber-Brigham and Women''s Cancer Center15. PCR methods for infectious disease diagnosis Richard Rothman, Padmini Ramachandran, Andrew Hardick, Charlotte Gaydos, Samuel Yang, all at the Department of Emergency Medicine, Johns Hopkins University16. Use of PCR for DNA methylation analysis Mario F.Fraga and Manel Esteller, both at the Spanish National Science Centre17. PCR-based methods to determine DNA methylation status at specific CpG sites using methylation-sensitive restriction enzymes Helmtrud Roach and Ko Hashimoto, both at the Bone and Joint Research Group, Southampton General Hospital18. PCR-based whole genome amplification Simon Hughes, Tumour Biology Laboratory, Cancer Research UK Clinical Centre, London; Nona Arneson and Susan Done, both at the Division of Experimental Therapeutics, Ontario Cancer Institute, Toronto19. PCR sequencing of human genes for the discovery of DNA sequence variants Abizar Lakdawalla, Applied BiosystemsList of suppliers; indexHughes, Simon is the author of 'PCR', published 2007 under ISBN 9781904842293 and ISBN 1904842291.